《植物生理学报》 2016, 52(5): 755-761

苹果果实可溶性固形物含量相关基因的全基因组筛选与分析

苑克俊^*, 程来亮, 牛庆霖, 王江勇

山东省果树研究所, 山东泰安271000

通信作者：苑克俊；E-mail: yuankj@vip.sina.com

摘　要：

可溶性固形物含量是苹果果实品质的重要指标。在果实成熟期对苹果杂交后代的果实可溶性固形物含量进行测定, 从中选择6个可溶性固形物含量低和4个含量高的植株分成2组, 在转录组水平上对其果实的基因表达进行比较分析。结果筛选出14个差异表达基因, 其中包括蛋白激酶基因和甲基转移酶基因, 表明蛋白质磷酸化和甲基化可能和苹果果实可溶性固形物含量相关。另外, 差异表达基因还包括转录因子、细胞色素b₆-f复合体亚单元7、形成素类蛋白和1-脱氧-D-木酮糖-5-磷酸合成酶等基因。这些结果为通过深入研究找出影响苹果果实可溶性固形物含量的关键基因提供了线索, 苹果果实可溶性固形物含量和上述这些基因的关系值得进一步研究。本研究还初步证实了基于转录组测序技术的数量性状集群分离分析法是可行的。

关键词：苹果; 果实; 可溶性固形物; 转录组; 基因

收稿：2016-02-25   修定：2016-04-28

资助：山东省泰山学者建设工程专项经费。

Genome-wide screen and analysis of the genes related to soluble solids content in apple fruits

YUAN Ke-Jun^*, CHENG Lai-Liang, NIU Qing-Lin, WANG Jiang-Yong

Shandong Institute of Pomology, Tai’an, Shandong 271000, China

Corresponding author: YUAN Ke-Jun; E-mail: yuankj@vip.sina.com

Abstract:

Soluble solids content in fruits is an important apple quality factor, it is very important to have a better understanding of the genes related to soluble solids content in apple fruits. In order to make clear whether there are the genes related to soluble solids content in apple fruits, one group of 6 hybrid plants with low and another group of 4 hybrid plants with high content of soluble solids were selected, a genome-wide transcriptome analysis was applied to analyze the gene expression of the fruits from these 10 hybrid plants. Comparative analysis of the gene expression profile revealed 14 significant differentially expressed genes between two groups of hybrid plants. Among them, some genes were recognised as protein kinase and methyltransferase, indicating that there are protein phosphorylation and methylation in apple fruits. In addition, transcription factor, cytochrome b₆-f complex subunit 7, formin-like protein, 1-deoxy-D-xylulose-5-phosphate synthase and some other genes were also recognised. These results may provide some help for further studies and some clues for screening key genes related to soluble solids content in apple fruits. Further research is needed to test the role of these 14 genes. Our results also confirmed the feasibility of bulk segregation analysis in analysing quantitative traits based on transcriptome sequencing.

Key words: apple; fruit; soluble solids; transcriptome; gene